Kenji Tsuge is a Japanese synthetic biologist famous for large-sized DNA manipulation using Bacillus subtilis. He is the inventor of OGAB method that can assembly multiple DNA fragments very efficiently using B. subtilis. He received PhD degree from Tokyo Institute of Technology, Japan in 1998. In 1999, he took part in Mitsubishi Kagaku Institute of Lifesciences, Japan, as Postdoctoral fellow. In 2006, he joined Institute for Advanced Biosciences, Keio University, Japan, as an Assistant Professor. In 2016, he moves to Kobe University as Associate Professor. During his research career, he has been studying genetic manipulation of B. subtilis, and invented several type of DNA manipulation methods that makes it possible to construct large sized DNA. His current research interest includes how to design functional genome from gene scratches. Now he is developing laboratory automation system for high throughput construction of long DNA.