Keiji Nishida is a Professor in the Graduate School of Science, Technology and Innovation, Kobe University, and the Deputy Director of Engineering Biology Research Center (EGBRC). Prof. Nishida have been interested in evolutionary design of life. My research career began with studying mitochondrial evolution and endosymbiosis with emphasis on the molecular mechanism of how mitochondrial division and segregation is conducted. In an attempt to integrate synthetic biology approach into evolutionary biology, I have demonstrated the emergence of new biological functionality by conferring bio-magnetism to yeast cells. These experiences lead me to realize the need for new technologies that accelerate designing of life system. My current research involves development of new genome editing tools that employs various DNA modification pathways to facilitate semi-rational and evolutionary design of life. The newly developed genome editing tool termed Target-AID enabled base-editing of DNA without cleavage and now is applicable to wide range of organisms.